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KMID : 0900919960200010071
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Korean journal of Animal Reproduction
1996 Volume.20 No. 1 p.71 ~ p.76
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Developmental Ability of Bovine Embryos Matured and Fetilized In Vitro after Freezing and Thawing to Gastrulation
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Lee Myung -Shik
Oh S.-J.
Yang B.-S.
Baek Kwang-Soo
Park Y.-Y.
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Abstract
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This experiment was carried out to investigate the developmental ability of bovine embryos matured and fertilized in vitro to the gastrulation stage. The bovine oocytes were collected from 2∼5mm follicles, matured for 20∼24hrs in 5% CO2 incubator and then fertilized with frozen-thawed semen. On day 9 after IVF and after freezing and thawing the hatching abilities of expanding blastocysts were examined. Cleavage rate and production rate to expanding blastocysts were 59.7%(955/1604) and 20.7%(333/1604), respectively. Hatching rate of day-9 expanding blastocysts was 54%(40/74), that after freezing and thawing was 56%(79/141). Also, developmental ability of hatched blastocysts to the primitive streak stage was 26%(6/23).
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KEYWORD
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Bovine, IVF embryos, frozen-thawed embryos, hatch, primitive streak stage
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